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Description: The Elite™ Acetylcholinesterase Assay Kit uses the outstanding Thiolite™ Green to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE in blood, in cell extracts, and in other solutions. Thiolite™ Green is not fluorescent until reacted with a thiol group. It has spectral properties similar to those of fluorescein, making this assay compatible with almost every fluorescence instrument. The fluorescence intensity of Thiolite™ Green is used to measure AChE activity. Compared to the existing thiol probes (e.g., mBBr and bBBr), Thiolite™ Green is much more sensitive.

Application: The Elite™ Acetylcholinesterase Assay Kit provides an ultrasensitive fluorometric one-step assay to detect as little as 0.01mU AChE in a 100 μl assay volume (0.1 mU/ml). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm.

Description: The Elite™ Aldehyde Detection kit is used for quantifying aldehydes at higher pH. This kit uses a proprietary fluorogenic dye that generates a strongly fluorescent product upon reacting with an aldehyde. This fluorimetric kit provides a sensitive mix-and-read method to detect as little as 0.3 nanomole of aldehyde in a 100 μl assay volume (3 μM).

Application: The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be read by a fluorescence microplate reader at Ex/Em = 365/435 nm.

Description: The Elite™ Glutathione Assay Kit provides an ultrasensitive fluorimetric assay to quantitate GSH in sample. The proprietary non-fluorescent glutathione sensor used in the kit becomes strongly green fluorescent upon reacting with a GSH compound, which has the spectral properties almost identical to those of fluorescein and can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm. The kit can detect as little as 1 picomole of GSH in a 100 μL assay volume (10 nM). In addition, both absorption and emission spectra of the glutathione adduct are pH-independent, making this assay kit highly robust.

Application: The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.

Description: There are quite a few reagents or assay kits available for quantitating thiols in biological systems. However, all the commercial kits either lack sensitivity or have tedious protocols. The Elite™ Glutathione GSH/GSSG Ratio Assay Kit provides an ultrasensitive assay to quantitate GSH in the sample. The kit uses a proprietary non-fluorescent dye that becomes strongly fluorescent upon reacting with GSH. With a one-step fluorimetric method, the kit can detect as little as 1 picomole of GSH or GSSG in a 100 μl assay volume.

Application: The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm.

Description: This kit uses our non-peptide HDAC Green™ substrate that is much more sensitive than the peptide-based HDAC substrates such as Ac-RGK(Ac)-R110, Ac-RGK(Ac)-AMC and Ac-RGK(Ac)-AFC. In addition, HDAC Green™ substrate is also much more resistant to protease hydrolysis than other commercial peptide-based HDAC substrates.

Application: It can be used for measuring HDAC activity in cell lysates or HDAC inhibitor screening with cell extracts or purified enzymes.

Ex/Em: 490/520 nm.

Description: The Elite™ Fluorimetric NADH Assay Kit provides a sensitive, one-step assay to detect as little as 100 pico-moles of NADH in a 100 μl assay volume (1 μM). It has very low background since it is run in the red visible range that significantly reduces the interference resulted from biological samples.

Application: The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm.

Ex/Em: 540/590 nm.

Description: The Elite™ Fluorimetric NAD/NADH Assay Kit provides a sensitive, one-step assay to detect as little as 10 pico-moles of NAD(H) in a 100 μl assay volume (100 nM). It has very low background since it is run in the red visible range that significantly reduces the interference resulted from biological samples.

Application: The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation without a separation step. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 530-570 nm/590-600 nm (maximum Ex/Em = 540/590 nm) or an absorbance microplate reader at ~576 nm.

Ex/Em: 540/590 nm.

Description: The Elite™ Fluorimetric NAD/NADH Ratio Assay Kit provides a convenient method for sensitive detection of NAD, NADH and their ratio; there is no need to purify NAD/NADH from sample mix. It has very low background since it is run in the red visible range that significantly reduces the interference resulted from biological samples.

Application: The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 530 - 570/590 - 600 nm (maximum Ex/Em = 540/590 nm) or an absorbance microplate reader at ~576 nm. This kit provides NAD and NADH extraction buffer, and cell lysis buffer for your convenience. It has been frequently used for determining NAD/NADH from cell lysates.

Ex/Em: 540/590 nm.

Description: The Elite™ Fluorimetric NADPH Assay Kit provides a sensitive, one-step assay to detect as little as 100 picomoles of NADPH in a 100 μl assay volume (1 μM). It has very low background since it is run in the red visible range that significantly reduces the interference resulted from biological samples.

Application: The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm.

This kit has been used for screening enzyme activities that use NADP/NADPH as a cofactor. It has also been used for the sensitive detection of NADPH in cell-based assays. Compared to the other commercial kits, this assay has higher signal/background ratio.

Ex/Em: 540/590 nm.

Description: The Elite™ Fluorimetric NADP/NADPH Assay Kit provides a sensitive, one-step assay to detect as little as 1 picomoles of NADPH in a 100 μl assay volume (10 nM). It has very low background since it is run in the red visible range that significantly reduces the interference resulted from biological samples.There is no need to purify NADP/NADPH from sample mix.

Application: The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm.

Ex/Em: 540/590 nm.

Description: This Elite™ Fluorimetric NADP/NADPH Ratio Assay Kit provides a convenient method for sensitive detection of NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme recycling reaction that significantly increases detection sensitivity. In addition, this assay has very low background since it is run in the red visible range that considerably reduces the sample interference.

Application: The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm.

Ex/Em: 540/590 nm.

Description: Elite™ cAMP ELISA Assay Kit provides the sensitive method for detecting adenylate cyclase activity in biochemical or cell-based assay system. Compared to other ELISA cAMP assay kits, our kit eliminates the tedious acetylation step. The kit uses Amplite™ Red as a fluorimetric substrate to quantify the HRP activity.

Application: The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. The fluorescent product formed is proportional to the activity of HRP-cAMP conjugate.

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Description: It provides all the essential components with an optimized assay protocol. It is designed to detect cell apoptosis by measuring generic activation of caspases (caspase-1, -3, -4, -5, -6, -7, -8 and -9) in live cells.

Application: Used for either the quantification of most activated caspase activities in apoptotic cells or screening of caspase inhibitors.

Ex/Em: 488/520 nm.

Description: This Kit uses (Z-DEVD)2 R110 as fluorogenic indicator for assaying caspase 3/7 activity. R110-derived caspase substrates are probably the most sensitive indicators used for the fluorimetric detection of various caspase activities.

Application: It can be used to continuously measure the activities of caspase 3/7 in cell extracts and purified enzyme preparations with a fluorescence microplate reader or fluorometer.

Ex/Em: 490/520 nm.

Description: Caspase 8 is proven to have substrate selectivity for the peptide sequence Ile-Glu-Thr-Asp (IETD). This kit uses (Ac-IETD)2-R110 as a fluorogenic indicator for caspase 8 activity. Cleavage of rhodamine 110 (R110) peptides by caspase 8 generates strongly fluorescent R110 which is monitored at the emission between 520 nm and 530 nm with the excitation between 480 nm and 500 nm. This spectral feature makes the kit compatible with the FITC filter set.

Application: The Elite™ Caspase 8 Assay Kit provides all the essential components with an optimized assay protocol. The assay can be readily adapted for high throughput screenings. It can be used to either quantify the activated caspase 8 activities in apoptotic cells or screen the caspase 8 inhibitors.

Description: Caspase 9 is proven to have selectivity for the peptide sequence Leu-Glu-His-Asp (LEHD). This kit uses (Ac-LEHD)2-R110 as a fluorogenic indicator for caspase 9 activity. Cleavage of R110 peptides by caspase 9 generates strongly fluorescent rhodamine 110 (R110) which is monitored at the emission between 520 nm and 530nm with the excitation between 480 nm and 500 nm.

Application: The Elite™ Caspase 8 Assay Kit provides all the essential components. The assay is robust and can be readily adapted for high throughput screening. It can be used to either quantify the activated caspase 9 activities in apoptotic cells or screen the caspase 9 inhibitors. Quite a few labs have used this kit for high throughput screening.

Description: It is more sensitive for cell proliferation and cytotoxicity than other assays such as MTT. The kit components are quite stable with minimal cytotoxicity, thus a longer incubation time (such as 24 to 48 hours) is possible if required.

Application: Excellent for high throughput screening of cell proliferation or cytotoxicity against a variety of compounds.

Ex/Em: 540/590 nm.

Description: Compared to Annexin V, the Apopxin™ PS sensor used in this kit is reengineered to have a much higher affinity to PS with Kd < 10 nM. The PS sensor has green fluorescence upon binding to membrane PS. The fluorescent sensor has Ex/Em = 490/520 nm, making assay readily run with FITC filter set.

Application: It can be used with a fluorescence microplate reader besides the microscope and flow cytometry platforms. HTS applications.

Ex/Em: 490/520 nm.

Description: Compared to Annexin V, the Apopxin™ PS sensor used in this kit is reengineered to have a much higher affinity to PS with Kd < 10 nM. The PS sensor has green fluorescence upon binding to membrane PS. The fluorescent sensor has Ex/Em = 490/520 nm, making assay readily run with FITC filter set.

Application: It is optimized for flow cytometric applications

Ex/Em: 490/520 nm.

Description: This kit is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Green™ LCS1 in permeabilized and fixed cells.

Application: It is optimized for flow cytometric applications.

Ex/Em: 490/520 nm.

Description: This kit uses a proprietary green fluorescent dye that easily permeates intact live cells and gets enhanced fluorescence upon entering into live cells. The hydrolysis of the non-fluorescent substrate by intracellular esterases generates a strongly green fluorescent hydrophilic product that is well-retained in the cell cytoplasm.

Application: It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit is suitable for proliferating and non-proliferating cells.

Ex/Em: 490/520 nm.

Description: This Kit is designed to uniformly label live cells with a non-fluorescent Calcein Green™ that becomes strongly fluorescent upon entering into live cells.

Application: It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity.

Ex/Em: 490/520 nm.

Description: The Elite™ Cell Assay Kit is designed to gauge cell growth by measuring proportional changes in total cell ATP. This is a homogenous assay, which can be performed on multi-well plates by adding the reagents directly to cell growth medium in both adherent and suspension cell cultures.

Application: This assay has been optimized to accurately measure cell number over a broad dynamic range (102 - 2x105 cells) in 96-well plate format, ideal for automated high-throughput screening. The assay accurately monitors cell growth over time periods from 24 – 120 h, and the ATP detection sensitivity is from 0.1 to 1000 µM. For cell proliferation, cell toxicity, and ATP measurement.

Description: Elite™ cAMP ELISA Assay Kit provides the sensitive method for detecting adenylate cyclase activity in biochemical or cell-based assay system. Compared to other ELISA cAMP assay kits, our kit eliminates the tedious acetylation step. The kit uses Amplite™ Red as a fluorimetric substrate to quantify the HRP activity.

Application: The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. The fluorescent product formed is proportional to the activity of HRP-cAMP conjugate.

Description: Mitochondrial membrane potential (Δψm) is an important parameter of mitochondrial function used as an indicator of cell health. EliteTM Mitochondrial Membrane Potential Detection Kit provides all the necessary reagents for the analysis of mictochondrial integrity in cells. The aggregate red form has absorption/emission maxima of 585/590 nm. The green monomeric form has absorption/ emission maxima of 510/527 nm.

Application: The assay is applicable to flow cytometry, fluorescence microscopy, and 96-well plate formats.