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Image Fusion
Delta2D's image fusion lets you combine several gel
images to one, new "ideal" gel. You can use Union fused
images, containing all spots of all combined images, for
making proteome maps, or Average fused images as a summary
of groups of replicate gels.
The full text of the article that describes Delta2D's
image fusion and its use for creating proteome maps is
freely available online:
Luhn S, Berth
M, Hecker M, Bernhardt J:
Using standard positions and image fusion to create proteome
maps from collections of two-dimensional gel electrophoresis
images.
PROTEOMICS 2003 Jul;3(7):1117-27
Digital Object Identifier (DOI):
10.1002/pmic.200300433
PubMed Entry:
PMID: 12872213
Related Features and Benefits
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Combine images
Delta2D's proprietary image fusion lets you
create proteome maps from collections of 2D
gel images. A proteome map looks like a real gel but
shows all spots on a single image — even those that
will never be expressed at the same time. You can
collect protein identifications on the map and then
use Delta2D to automatically and reliably transfer
them to new gels. Gel images can also be merged into
an average image which combines replicates
pixel-by-pixel. Read more about
Proteome Maps. |
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Color spots according to their expression
profiles.
The larger an experimental design, the higher the
analytical complexity. Spot color coding
condenses whole experiment expression profiles by
using different colors in just one gel image. This
simplifies extraction of the really interesting
spots. |
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Standardized Spot Shapes due to Spot Transfer
Spot boundaries detected on union fusion images
which contain the image information of a whole 2D
gel study can be transferred to every gel of your
experiment by using the Transfer Spots
procedure of Delta2D. This results in uniform spot
shapes over the whole experiment, generates complete
expression profiles and prevents for the classical
problems of multiple matches known from traditional
2D gel analysis packages. |
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