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Analyze All Kinds of Multiplex Experiments

The new Delta2D supports electrophoresis technologies that generate multiple images per gel. One of these is DIGE (Fluorescence 2D Difference Gel Electrophoresis), where multiple samples are labeled using different fluorescence stains and then comigrating on a single gel. For DIGE experiments, Delta2D supports all experimental setups, including those using an internal standard.

Another application of Delta2D is analyzing images generated using Glycoprotein and Phosphoprotein Gel Stains (Molecular Probes, Eugene, Oregon).

Related Features and Benefits

 

Manage collections of gel images.

All relevant data are kept in a pool: images, labels, spot data etc. You can group gels and combine groups to projects. Analysis can be done gel vs. gel or group vs. group. Delta2D's convenient project table helps you organize it all.

 
Compare many samples simultaneously

The Regions View displays the same region of all samples in the same, freely choosable zoom factor. All regions are kept in sync when you move them around.

 
Color spots according to their expression profiles.

The larger an experimental design, the higher the analytical complexity. Spot color coding condenses whole experiment expression profiles by using different colors in just one gel image. This simplifies extraction of the really interesting spots.

 
Standardized Spot Shapes due to Spot Transfer

Spot boundaries detected on union fusion images which contain the image information of a whole 2D gel study can be transferred to every gel of your experiment by using the Transfer Spots procedure of Delta2D. This results in uniform spot shapes over the whole experiment, generates complete expression profiles and prevents for the classical problems of multiple matches known from traditional 2D gel analysis packages.

 

Delta2DWhat's New in v4.0
Analysis Workflow
Compact Windowing
Light Table
Project Explorer
Warping Setup
Image Processing
Qualitative Analysis
Quantitative Analysis
Image Fusion
Proteome Maps
Multiplex Experiment
Scouts
Detailed Info
Tech Specifications
Guided Tour
Evaluation Version
FAQ
Reference List

 

     
 

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