What's New in v4.0

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With Delta2D's new user interface you can analyze 2D gel experiments faster and easier than ever.

The Workflow guides you through the analysis.	New Compact Windowing lets you arrange tables, images, and more.	Use the Light Table to organize the gel images in your project.
See the project and all analysis data at a glance with the Project Explorer.	The new Warping Setup makes it easier than ever to use the warping strategy that is most appropriate for your needs.

Numerous other improvements in performance, web reports, and other areas:

Statistics

We have introduced four nonparametric tests from the TMeV Nonpar module. These tests have the advantage that they do not assume a normal distribution for spot quantities.

Wilcoxon Rank Sum Test
for testing one factor in two experimental groups.

Kruskal-Wallis Test
for testing one factor in multiple experimental groups. This is a generalization of the Wilcoxon rank sum test.

Mack-Skillings Test
for testing two factors in multiple experimental groups. You can use this as a distribution-free alternative to the classic two-way ANOVA.

Fisher Exact Test
for testing nonrandom associations between two categorical variables.

The meaning of colors in the t-test column of Delta2D's statistics table was made more consistent: green indicates high confidence (low p-values).

Operations on sets of gel images

When we introduced the new user interface we also made some operations more consistent and intuitive. For example, you can now select a gel image pair in a window and choose Scatter Plot from its context menu.

Wherever you can select multiple gel images (for example in the Project Explorer), you can now open a quantitation table that shows just these images. Furthermore, you can open multiple quantitation tables, such as one table for each replicate group, side-by-side.

Improved Image Fusion and Spot Transfer

Image fusion and spot transfer are now controlled by similar dialogs. As with many other operations, you can select multiple images in the Project Explorer and fuse them. Similarly, you can select images that should become the target of a spot transfer.

Improved Performance

Delta2D 4.0 introduces substantial performance improvements in large projects, for operations such as filtering or hiding spots. These improvements were also made available in the most recent updates of Delta2D 3.6.

Web Reports

You can now switch spot boundaries on or off in reports, we have added mean and RSD columns to expression profiles. The dual channel version of the spot album report now displays the reference image in a dual channel version.

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