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Nucleotides and Modified Nucleotides

bulletdNTPs Set (dATP, dTTP, dCTP, dGTP)
bulletdNTP(GAC)/dUTP set
bulletBiotin-11-dUTP
bulletAZTTP
bulletAA-dUTP
bulletRhodamine-5-dUTP (Rho-5-dUTP)
bulletFluorescein-12-dUTP
bulletBrdUTP
bullet8-oxo-dGTP
bullet7-deaza-dGTP

 

dNTPs Set (dATP, dTTP, dCTP, dGTP)
for PCR amplification, 4 x 25 µmol 

bulletDescription: Ready-to-use dNTP (dATP, dTTP, dCTP, dGTP) solutions are available as tetrasodium salts for use in DNA polymerization reactions and all DNA labeling and sequencing processes.
bulletPack size:100 mM each solution, 4 x 25 µmol each, 250 µl each vial, can prepare 1 ml 25 mM dNTPs solution.
bulletQuality Control:
bulletQuality controlled by HPLC for maximum purity and minimal di- and monophosphate content;
bulletPurity of >98% by HPLC;
bulletFunctionally tested in long template PCR amplification and standard labeling reactions.
bulletStorage: Store at -20°C.

 

Part #

Description

Price ($US)

Quantity

NT-013-0050 dNTPs Set (dATP, dTTP, dCTP, dGTP) $129.00


- Ordering Instructions -

 

dNTP(GAC)/dUTP set
4 x 25 µmol  

bulletDescription: Ready-to-use dNTP (dATP, dUTP, dCTP, dGTP) solutions are available as tetrasodium salts for use in DNA polymerization reactions and all DNA labeling and sequencing processes.
bulletPack size:100 mM each solution, 4 x 25 µmol each, 250 µl each vial, can prepare 1 ml 25 mM dNTPs solution.
bulletQuality Control:
bulletQuality controlled by HPLC for maximum purity and minimal di- and monophosphate content;
bulletPurity of >98% by HPLC;
bulletFunctionally tested in long template PCR amplification and standard labeling reactions.
bulletStorage: Store at -20°C.

 

Part #

Description

Price ($US)

Quantity

NT-043-0050 dNTP(GAC)/dUTP set
100 mM, 4 x 25 µmol
$149.00


- Ordering Instructions -

 

Biotin-11-dUTP
1 mM (50 nmol) 

bullet

Application
bullet

Enzymatic non-radioactive labeling of DNA in PCR, nick-translation, cDNA synthesis or primer extension reactions.

bullet

Can be incorporated into DNA using Terminal Deoxynucleotididyl Transferase, Reverse Transcriptases, Taq DNA Polymerase, Klenow Fragment.

 

bulletDescription: Biotin-11-dUTP (biotin-e-aminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5'-triphosphate]) is supplied as a 1 mM aqueous solution at pH 7.0.
bulletStorage: Stable at -20°C for at least 6 month.
bullet

Diluent: dd-H2O

bullet

Concentration: 50 nmol (50 µl of 1 mM solution)

bullet

Formula: C28H41N6O17P3SLi4,  Molecular Weight (886.5)

 

 

Part #

Description

Price ($US)

Quantity

BU-013-0115 Biotin-11-dUTP
1 mM (50 nmol)
$129.00


- Ordering Instructions -

AZTTP
1 µmol (100 µl of 10 mM solution) 

bulletDescription: 3′-Azido-3′-Deoxythymidine-5′-Triphosphate
AZT is a widely used antiviral agent. AZT penetrates the cell membrane and is converted into AZT triphosphate which inhibits the reaction of reverse transcription. AZT triphosphate can be used for inhibition of reverse transcription in vitro and for the investigation of polymerase-substrate interactions.
bulletStorage: -20°C
bullet

Concentration: 1 µmol (100 µl of 10 mM solution)

bullet

Purity: 96-99% (HPLC)

bullet

Formula: C10H13N5P3O13, MW 504.2

 

 

Part #

Description

Price ($US)

Quantity

AT-013-0170 AZTTP
1 µmol (100 µl of 10 mM solution)
$69.00


- Ordering Instructions -

 

AA-dUTP
1 µmol (100 µl of 10 mM solution)  

bulletDescription: 5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate
AA-dUTPcan be used for enzymatic indirect non-radioactive labeling of DNA in PCR, nick-translation, cDNA synthesis or primer extension reactions. It can be incorporated into DNA using Reverse Transcriptases, Taq DNA Polymerase, Klenow Fragment. The resulting aminoallyl-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten, resulting in a uniform and high density labeling of DNA.
bulletStorage: -20°C
bullet

Concentration: 1 µmol (100 µl of 10 mM solution)

bullet

Purity: 96-99% (HPLC)

 

 

Part #

Description

Price ($US)

Quantity

AU-013-0140 AA-dUTP
1 µmol (100 µl of 10 mM solution)
$59.00


- Ordering Instructions -

 

Rhodamine-5-dUTP (Rho-5-dUTP)
50 nmol (50 µl of 1 mM solution)  

bulletDescription: Tetramethylrhodamin-5(6)-[5-(3-carboxaminoallyl) 2'-deoxyuridine 5'-triphosphate].
Fluorescent labeling of DNA by terminal deoxynucleotidyl transferase or DNA-polymerases.
bulletStorage: -20°C
bullet

Concentration: 50 nmol (50 µl of 1 mM solution)

bullet

Purity: 96-99% (HPLC)

 

 

Part #

Description

Price ($US)

Quantity

RU-013-0135 Rhodamine-5-dUTP(Rho-5-dUTP)
50 nmol (50 µl of 1 mM solution)
$199.00


- Ordering Instructions -

 

Fluorescein-12-dUTP
50 nmol (50 µl of 1 mM solution) 

bulletDescription: Fluorescein-5(6)-carboxamidocaproyl-[5-(3-aminoallyl)-2′- deoxyuridine 5′-triphosphate]
Flu-12-dUTP is used for the fluorescent labelling of DNA by terminal deoxynucleosidtransferase or DNA-polymerase.
bulletStorage: -20°C
bullet

Concentration: 50 nmol (50 µl of 1 mM solution)

bullet

Purity: 96-99% (HPLC)

 

Part #

Description

Price ($US)

Quantity

FU-013-0125 Fluorescein-12-dUTP
50 nmol (50 µl of 1 mM solution)
$199.00


- Ordering Instructions -

 

BrdUTP
1 µmol (100 µl of 10 mM solution)

bulletDescription: 5-Bromo-2′-Deoxyuridine-5′-Triphosphate
BUdR triphosphate can be used for incorporating into DNA for the subsequent detection with anti-BUdR antibodies.
BUdR triphosphate incorporation into DNA is also a tool for random-mutation introduction.
bulletStorage: -20°C
bullet

Concentration: 1 µmol (100 µl of 10 mM solution)

bullet

Purity: 96-99% (HPLC)

 

 

Part #

Description

Price ($US)

Quantity

Bu-013-0180 BrdUTP
1 µmol (100 µl of 10 mM solution)
$59.99


- Ordering Instructions -

 

8-oxo-dGTP

bulletDescription: 8-oxo-2′-deoxyguanosine-5′-triphosphate
bulletFormula: C10H12N5O14P3
MW 519.15 (Anion)
bulletStorage: -20°C
bullet

Concentration: 1 µmol (100 µl of 10 mM solution)

bullet

Purity: 96-99% (HPLC)

 

 

Part #

Description

Price ($US)

Quantity

OG-013-0150 8-oxo-dGTP $199.00


- Ordering Instructions -

 

7-deaza-dGTP
1 µmol (100 µl of 10 mM solution)

bulletDescription: 7-deaza-2′-deoxyguanosine-5′-triphosphate
7-deaza-dGTP may be effectively incorporated into the DNA by DNA polymerases and/or terminal deoxynucleotidyl transferase. 7-deaza-dG - containing DNA duplexes demonstrate increased stability, 7-deaza-dG - containing DNA penetrates cell membrane more effectively due to the increased lypophility.
7-deaza dGTP may be used in sequencing for the resolution of the compressions in G-C-rich regions. Amplification of some DNA regions is often hindered due to the high G-C content. Replacement of dGTP to 7-deaza dGTP in the master mix may solve the problems.
bulletFormula: C11H17N4O13P3,  Molecular Weight (506.2)
bulletStorage: -20°C
bullet

Concentration: 1 µmol (100 µl of 10 mM solution)

bullet

Purity: 96-99% (HPLC)

 

Part #

Description

Price ($US)

Quantity

DG-013-0160 7-deaza-dGTP
1 µmol (100 µl of 10 mM solution)
$99.00


- Ordering Instructions -

 

 

 

 

 

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