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>> Download KlenThermase DNA Polymerase datasheet
2 x HotStart PCR Master Mix
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 | Description:
HotStart PCR
Ready Mix is a 2 x concentrated reagent mix for Hot Start PCR
comprising two cold sensitive mutant Taq polymerases, 2 x PCR-buffer
with 5 mM MgCl2, 400µM of each dNTP and stabilizers. Unlike
monoclonal antibody based hot start Taq or chemical modified hot
start Taq, cold sensitive mutant Taq will retain the hot start
ability throughout the whole amplification cycles.
Cold sensitive mutant Taq polymerases are designed for "Hot Start" PCR due to their suppressed activity at low temperature. It offers excellent specificity and two-fold higher fidelity than wild-type Taq. It is designed for PCR with difficult templates such as GC-rich fragments and microsatellites. Cold sensitive mutant Taq polymerases are particularly well suited to primer extension of Single Nucleotide Polymorphism (SNP) markers.
Cold sensitive mutant Taq polymerases maintain excellent specificity and minimal background even in conditions designed for high yield (high Mg2+ /primer concentrations). In fact, even on genomic templates, the enzyme can be used with MgCl2+ concentrations as high as 10 mM.
Cold sensitive mutant Taq polymerases are capable of extending through difficult regions, e.g. regions, which include inverted tandem repeats and those with high amounts of secondary structure.
Cold sensitive mutant Taq polymerases work in a totally unique way, involving improved nucleotide selection at the active site, and a much lower rate of mis-match extension, meaning that only perfectly aligned primers will be extended. As a result, the enzyme can give even higher specificity than hot-start (manual or automatic) techniques without the need for inconvenient pre-incubation steps.
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| Application:
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| Quality Control: Activity, non-specific endonucleases, nickases, and exonucleases. |
| Storage: Recommended to keep at 2-8°C for immediate use (12 month) and -20°C for long term storage. |
>> Download PCR Master Mix datasheet
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Part # |
Description |
Price ($US) |
Quantity |
| DP-016-0250 | Hot Start PCR Ready Mix (2x), monoclonal antibody based hot
start 250 rxns (20 µl each) |
$149.00 | |
| DP-016-1000 | Hot Start PCR Ready Mix (2x), monoclonal antibody based hot
start 1000 rxns (20 µl each) |
$516.00 |
| Description:
The one-step RT-PCR kit contains all necessary reagents for high
specificity first strand cDNA synthesis and subsequent PCR
amplification. TthPlus DNA polymerase is isolated from the
Thermus thermophilus strain. TthPlus DNA polymerase is a single
92 kDa polypeptide showing a 5′-3′ exonuclease activity but
lacking 3′-5′ exonuclease activity. It catalyzes the
polymerization of nucleotides into double-stranded DNA in the
presence of MgCl2. Its efficiency has been shown more
particularly on large DNA fragments up to 12 kb (using lambda
phage DNA as a template). TthPlus DNA polymerase is also capable
of catalyzing the polymerization of DNA using a RNA template in
the presence of Mn2+. The ability of TthPlus DNA polymerase to
reverse transcribe at elevated temperatures (70°C) minimizes the
problems encountered with strong secondary structures in RNA
since they are unstable at higher reaction temperatures. Higher
temperatures also result in increased specificity of primer
hybridization and extension. In coupled RT/PCR assays, TthPlus
is about 50-100 times more efficient than regular Taq DNA
polymerase.
The kit provides a one-tube 10X buffer for both reactions (RT and PCR) using the same enzyme (TthPlus). The 10 x one-tube buffer does not contain Mn(OAc). Mn(OAc) must be added at a final concentration of 3.5 mM if reverse transcription is required.
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| Application:
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| Storage: Store at -20°C, in a constant temperature freezer. | ||||||||||
| Components:
The optimal experimental conditions depend on the system used and they should be individually determined. The Mn2+ concentrations and the enzyme amount are the limiting factors for an accurate result. Normally 1.5-2.5 units of enzyme and a Mn-acetate concentration of 3.5 mM are used for the reverse transcription in a final 25 µl reaction
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Part # |
Description |
Price ($US) |
Quantity |
| MK-330-0200 | 200 rxns (25 µl each) | $229.00 |
| Description: Synergy Taq is a mix of thermostable polymerases possessing 5′-3′ DNA polymerase activity that is optimized to amplify longer GC-rich fragments and 3′-5′ proof-reading activity. These components are optimized to achieve amplification of 15 kb products from genomic DNA. Synergy Taq provides a very robust synthesis of longer GC-rich amplification products. | ||||
| Application:
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| Concentration: 10 units/µl |
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Unit Definition: One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-aminopropane-sulfonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCl, 2 mM MgCl2, 1 mM .-mercaptoethanol) and activated calf thymus DNA as substrate. |
| Storage buffer: 10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA; 1 mM DTT, 0.01% Tween 20; 50% glycerol (v/v). |
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Storage temperature: Store Synergy Taq DNA polymerase below 0°C, preferably at -20°C, in a constant temperature freezer. |
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10x reaction buffer: 160 mM (NH)4SO4, 670 mM Tris-HCl pH 9,1; 8% Glycerol, 2% DMSO, 35 mM MgCl2. |
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Quality control:Activity, non-specific endonucleases, nickases, and exonucleases. |
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Part # |
Quantity |
Price ($US) |
Quantity |
| DP-007-0010 | 100 units
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$42.00 | |
| DP-007-0050 | 500 units | $152.00 |
| Description:
TthPlus DNA polymerase is isolated from the Thermus thermophilus
strain. TthPlus DNA polymerase is a single 92 kDa polypeptide
showing a 5′-3′
exonuclease activity but lacking 3′-5′
exonuclease activity. It catalyzes the polymerization of
nucleotides into double-stranded DNA in the presence of MgCl2.
Its efficiency has been shown more particularly on large DNA
fragments up to 12 kb (using lambda phage DNA as a template).
TthPlus DNA polymerase is also capable of catalyzing the
polymerization of DNA using a RNA template in the presence of Mn2+.
The ability of TthPlus DNA polymerase to reverse transcribe at
elevated temperatures (70°C)
minimizes the problems encountered with strong secondary
structures in RNA since they are unstable at higher reaction
temperatures. Higher temperatures also result in increased
specificity of primer hybridization and extension. In coupled RT/PCR
assays, TthPlus is about 50-100 times more efficient than
regular Taq DNA polymerase.
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| Application:
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| Concentration: 5 units/µl | ||||||
| Features
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| Unit definition: One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the following reaction conditions: 25 mM TAPS buffer (Tris-(hydroxymethyl)-methyl- amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C), 50 mM KCl, 2 mM MgCl2, 1 mM β-mercaptoethanol, 200 µM dNTPs and 10 µg of calf thymus DNA in a final reaction volume of 50 µl. | ||||||
| Storage buffer: 10 mM K-phosphate buffer pH 7.0 (25°C), 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 50% glycerol (v/v), 0,1 mg/ml BSA. | ||||||
| Storage temperature: Store TthPlus DNA polymerase, preferably at -20°C, in a constant temperature freezer. | ||||||
| Component:
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>> Download TthPlus DNA Polymerase datasheet
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Part # |
Description |
Price ($US) |
Quantity |
| DP-015-0050 | $145.00 |
| Description: Mouse Monoclonal Antibody to Taq DNA Polymerase |
| Immunogen: Full length Taq DNA polymerase |
| Specificity: Reacts with various commercial available Taq DNA polymerases (native or recombinant) |
| Isotype: IgG2b |
| Clone number: 8C1 |
| Purity: › 95% by SDS PAGE gel |
| Size: 100 µg (4 µg/µl) |
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Part # |
Size |
Price ($US) |
Quantity |
| MA-029-0250 | 0.1 mg (25 µl) | $88.00 |
| Description: Monoclonal Anti-Taq Antibody, Glycerol-free |
| Immunogen: Full length Taq DNA polymerase |
| Specificity: Reacts with various commercial available Taq DNA polymerases (native or recombinant) |
| Applications: Keep the Taq DNA polymerase from activated at storage conditions; eliminate the possible inhibitory effect by glycerol in the PCR amplification |
| Isotype: IgG2b |
| Clone number: 8C1C |
| Purity: › 95% by SDS PAGE gel |
| Size: 100 µg (4 µg/µl) |
>> Download Glycerol-free Anti-Taq Monoclonal Antibody datasheet
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Part # |
Size |
Price ($US) |
Quantity |
| MA-039-0100 | 0.1 mg (25 µl) | $95.00 |
| Catalog | |
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