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Microplates Glass Plates Taq DNA Polymerase Assay Detection Kits Ready Stain Solution Molecular Biology Kits Nucleotides
  Taq DNA Polymerase

 

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Taq DNA Polymerase

bulletEU Taq DNA Polymerase, Highly Purified
bulletBioTherm Taq DNA Polymerase
bulletSupraTaq DNA Polymerase
bulletKlenThermase DNA Polymerase
bullet2 x HotStart PCR Master Mix
bulletOne-Tube RT-PCR System
bulletSynergy Taq DNA Polymerase
bulletTthPlus DNA Polymerase
bulletAnti-Taq Monoclonal Antibody (8C1)
bulletGlycerol-free Anti-Taq Monoclonal Antibody (8C1C)
 

EU Taq DNA Polymerase, Highly Purified

bulletDescription:
bullet

Highly purified thermostable DNA polymerase from the Thermus sp. strain;

bullet

No detectable bacterial DNA;

bullet

Half life of EU-Taq: 95 C x 3 hrs (normal Taq: 94 C x 45 mins);

bullet

Error rate: 1 x 10-6 compare to other Taqs of 1 x 10-4 to 2 x 10-5

bulletApplication:
 
bullet

RAPD-PCR

bullet

High-throughput genotyping/screening

bullet

Multiplexed genotyping

bullet

Quantitative Real-time PCR

bullet

Nested PCR

 

bulletBuffer (10x): 160 mM (NH 4 )2 SO4, 670 mM Tris-HCl pH 8.8 (at 25C),  0.1% Tween 20
bulletMgSO4: 20 mM
bulletConcentration: 5 U/l
bulletStorage: -20 C

>> Download EU Taq DNA Polymerase datasheet

 

Part #

Quantity

Price ($US)

Quantity

DP-005-0050

500 units

$65.00


- Ordering Instructions -

 

BioTherm Taq DNA Polymerase

Very robust Taq DNA Polymerase for routine PCR applications.

 

bulletDescription: Thermostable DNA polymerase purified from the Thermus aquaticus strain.
bulletApplication: Robust DNA amplification of plasmid or genomic DNA (100 bp-10kb).
bulletBuffer (10x): 160 mM (NH 4 )2 SO4, 670 mM Tris-HCl pH 8.8 (at 25C), 15 mM MgCl2, 0.1% Tween 20
bulletConcentration: 5 U/l
bulletStorage: -20 C

>> Download BioTherm Taq DNA Polymerase datasheet

 

Part #

Quantity

Price ($US)

Quantity

DP-002-0050 500 units $69.00


 

- Ordering Instructions -

 

SupraTaq DNA Polymerase

For routine PCR application.

 

bulletDescription: Native DNA polymerase purified from the Thermus aquaticus strain.
bulletApplication: Amplification of DNA fragments of 100 bp-5kb size.
bulletBuffer (10x): 160 mM (NH 4 )2 SO4, 670 mM Tris-HCl pH 8.8 (at 25C), 15 mM MgCl2, 0.1% Tween 20
bulletConcentration: 5 U/l
bulletStorage: -20 C

>> Download SupraTaq DNA Polymerase datasheet

 

Part #

Quantity

Price ($US)

Quantity

DP-001-0050 500 units $49.99


- Ordering Instructions -

 

KlenThermase DNA Polymerase
Optimized for DNA Sequencing.

bulletDescription: KlenThermase DNA polymerase is an optimized version of DNA polymerase designed for cycle sequencing with dideoxynucleotides.
bulletApplication: Designed for automated fluorescent DNA sequencing and for manual DNA sequencing with 35S label.
bulletBuffer (10x): 500 mM KCl, 100 mM Tris-HCl pH 9.0 (at 25C), 1% Triton X100, with 50 mM MgCl2
bulletConcentration: 25 U/l
bulletStorage: -20 C

 

>> Download KlenThermase DNA Polymerase datasheet

 

Part #

Quantity

Price ($US)

Quantity

DP-010-0010 1,000 units $150.00


- Ordering Instructions -

 

2 x HotStart PCR Master Mix
Hot Start function for all cycles of PCR amplification.

bulletDescription: HotStart PCR Ready Mix is a 2 x concentrated reagent mix for Hot Start PCR comprising two cold sensitive mutant Taq polymerases, 2 x PCR-buffer with 5 mM MgCl2, 400M of each dNTP and stabilizers. Unlike monoclonal antibody based hot start Taq or chemical modified hot start Taq, cold sensitive mutant Taq will retain the hot start ability throughout the whole amplification cycles.

 

Cold sensitive mutant Taq polymerases are designed for "Hot Start" PCR due to their suppressed activity at low temperature. It offers excellent specificity and two-fold higher fidelity than wild-type Taq. It is designed for PCR with difficult templates such as GC-rich fragments and microsatellites. Cold sensitive mutant Taq polymerases are particularly well suited to primer extension of Single Nucleotide Polymorphism (SNP) markers.

 

Cold sensitive mutant Taq polymerases maintain excellent specificity and minimal background even in conditions designed for high yield (high Mg2+ /primer concentrations). In fact, even on genomic templates, the enzyme can be used with MgCl2+ concentrations as high as 10 mM.

 

Cold sensitive mutant Taq polymerases are capable of extending through difficult regions, e.g. regions, which include inverted tandem repeats and those with high amounts of secondary structure.

 

Cold sensitive mutant Taq polymerases work in a totally unique way, involving improved nucleotide selection at the active site, and a much lower rate of mis-match extension, meaning that only perfectly aligned primers will be extended. As a result, the enzyme can give even higher specificity than hot-start (manual or automatic) techniques without the need for inconvenient pre-incubation steps.

 

bulletApplication:
bulletHot start PCR amplification of all various templates or tasks ;
bulletdesigned for amplification of difficult templates, such as GC-rich fragments and microsatellites;
bulletprimer extension of SNP markers;
bullet amplification of genomic DNA targets up to 10 kb with high fidelity, specificity, and sensitivity;
bullethigh through-put Hot Start PCR with high specificity, sensitivity, and yield;
bulletroutine diagnostic Hot Start PCR requiring high reproducibility.

 

bulletQuality Control: Activity, non-specific endonucleases, nickases, and exonucleases.
bulletStorage: Recommended to keep at 2-8C for immediate use (12 month) and -20C for long term storage.

>> Download PCR Master Mix datasheet

 

Part #

Description

Price ($US)

Quantity

DP-016-0250 Hot Start PCR Ready Mix (2x), monoclonal antibody based hot start

250 rxns (20 l each)

$149.00


DP-016-1000 Hot Start PCR Ready Mix (2x), monoclonal antibody based hot start

1000 rxns (20 l each)

$516.00


- Ordering Instructions -

 

One-Tube RT-PCR System
Sufficient for 200 Reactions

 

bulletDescription: The one-step RT-PCR kit contains all necessary reagents for high specificity first strand cDNA synthesis and subsequent PCR amplification. TthPlus DNA polymerase is isolated from the Thermus thermophilus strain. TthPlus DNA polymerase is a single 92 kDa polypeptide showing a 5′-3′ exonuclease activity but lacking 3′-5′ exonuclease activity. It catalyzes the polymerization of nucleotides into double-stranded DNA in the presence of MgCl2. Its efficiency has been shown more particularly on large DNA fragments up to 12 kb (using lambda phage DNA as a template). TthPlus DNA polymerase is also capable of catalyzing the polymerization of DNA using a RNA template in the presence of Mn2+. The ability of TthPlus DNA polymerase to reverse transcribe at elevated temperatures (70C) minimizes the problems encountered with strong secondary structures in RNA since they are unstable at higher reaction temperatures. Higher temperatures also result in increased specificity of primer hybridization and extension. In coupled RT/PCR assays, TthPlus is about 50-100 times more efficient than regular Taq DNA polymerase.

 

The kit provides a one-tube 10X buffer for both reactions (RT and PCR) using the same enzyme (TthPlus). The 10 x one-tube buffer does not contain Mn(OAc). Mn(OAc) must be added at a final concentration of 3.5 mM if reverse transcription is required.

 

bulletApplication:
bulletFor single tube RT-PCR and Real-Time Quantitative PCR;
bulletFor high temperature reverse transcription that allows highly specific priming of cDNA synthesis and overcomes problems with secondary structure.

 

bulletStorage: Store at -20C, in a constant temperature freezer.
bulletComponents:
bulletTthPlus DNA polymerase, 500 units
bullet10 x OneTube buffer, 1.5 ml
bullet50 mM Mn(OAc)2, 0.5 ml
bulletNucleotide Mix (10 mM dATP,
bullet10 mM dCTP, 10 mM dGTP, 20 mM dUTP), 0.25 ml
 

The optimal experimental conditions depend on the system used and they should be individually determined. The Mn2+ concentrations and the enzyme amount are the limiting factors for an accurate result. Normally 1.5-2.5 units of enzyme and a Mn-acetate concentration of 3.5 mM are used for the reverse transcription in a final 25 l reaction

 

Part #

Description

Price ($US)

Quantity

MK-330-0200 200 rxns (25 l each) $229.00


- Ordering Instructions -

 

 

Synergy Taq DNA Polymerase
For high fidelity amplification of long template DNA with condensed GC-rich sequences.

bulletDescription: Synergy Taq is a mix of thermostable polymerases possessing 5-3 DNA polymerase activity that is optimized to amplify longer GC-rich fragments and 3-5 proof-reading activity. These components are optimized to achieve amplification of 15 kb products from genomic DNA. Synergy Taq provides a very robust synthesis of longer GC-rich amplification products.
bulletApplication:
bullet

Synergy Taq has all the properties of regular Taq DNA Polymerases for every standard PCR application.

bullet

Optimized for high fidelity amplification of long template DNA with condensed GC-rich sequences; it can amplify up to 15 kb genomic DNA target and 35 kb lDNA sequence.

 

bullet Concentration: 10 units/l
bullet

Unit Definition: One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-aminopropane-sulfonic acid, sodium salt) pH 9.3 (at 25C), 50 mM KCl, 2 mM MgCl2, 1 mM .-mercaptoethanol) and activated calf thymus DNA as substrate.

bulletStorage buffer: 10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA; 1 mM DTT, 0.01% Tween 20; 50% glycerol (v/v).
bullet

Storage temperature: Store Synergy Taq DNA polymerase below 0C, preferably at -20C, in a constant temperature freezer.

bullet

10x reaction buffer: 160 mM (NH)4SO4, 670 mM Tris-HCl pH 9,1; 8% Glycerol, 2% DMSO, 35 mM MgCl2.

bullet

Quality control:Activity, non-specific endonucleases, nickases, and exonucleases.

 

 

Part #

Quantity

Price ($US)

Quantity

DP-007-0010 100 units

 

$42.00


DP-007-0050 500 units $152.00


- Ordering Instructions -


TthPlus DNA Polymerase
for One Step RT-PCR, 500 Units

bulletDescription: TthPlus DNA polymerase is isolated from the Thermus thermophilus strain. TthPlus DNA polymerase is a single 92 kDa polypeptide showing a 5-3 exonuclease activity but lacking 3-5 exonuclease activity. It catalyzes the polymerization of nucleotides into double-stranded DNA in the presence of MgCl2. Its efficiency has been shown more particularly on large DNA fragments up to 12 kb (using lambda phage DNA as a template). TthPlus DNA polymerase is also capable of catalyzing the polymerization of DNA using a RNA template in the presence of Mn2+. The ability of TthPlus DNA polymerase to reverse transcribe at elevated temperatures (70C) minimizes the problems encountered with strong secondary structures in RNA since they are unstable at higher reaction temperatures. Higher temperatures also result in increased specificity of primer hybridization and extension. In coupled RT/PCR assays, TthPlus is about 50-100 times more efficient than regular Taq DNA polymerase.


TthPlus DNA polymerase is delivered with a one-tube 10x buffer. Both reactions (RT and PCR) are carried out in the same buffer and same tube with the same enzyme (TthPlus).  The 10 x one tube buffer does not contain Mn(OAc)2. Mn(OAc)2 is provided extra and have to be added at a final concentration of 3.5 mM.

 

bulletApplication:
bullet

DNA polymerase having both reverse transcription and DNA amplification activity, excellent for one step RT-PCR and real-time quantitative PCR;

bullet

For high temperature reverse transcription that allows highly specific priming of cDNA synthesis and overcomes problems with secondary structure.

 

bulletConcentration: 5 units/l
bulletFeatures
bulletThermostable
bulletTaq DNA polymerase activity
bulletReverse transcriptase activity at 70 C
 
bulletUnit definition: One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72C under the following reaction conditions: 25 mM TAPS buffer (Tris-(hydroxymethyl)-methyl- amino-propanesulfonic acid, sodium salt) pH 9.3 (25C), 50 mM KCl, 2 mM MgCl2, 1 mM β-mercaptoethanol, 200 M dNTPs and 10 g of calf thymus DNA in a final reaction volume of 50 l.
bulletStorage buffer: 10 mM K-phosphate buffer pH 7.0 (25C), 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 50% glycerol (v/v), 0,1 mg/ml BSA.
bulletStorage temperature: Store TthPlus DNA polymerase, preferably at -20C, in a constant temperature freezer.
bulletComponent:
bulletTthPlus DNA polymerase
bullet10 x OneTube buffer
bullet50 mM Mn(OAc)2
 

>> Download TthPlus DNA Polymerase datasheet

 

Part #

Description

Price ($US)

Quantity

DP-015-0050   $145.00


- Ordering Instructions -

 


Anti-Taq Monoclonal Antibody (8C1)
250 Units (100 g) 

bulletDescription: Mouse Monoclonal Antibody to Taq DNA Polymerase
bulletImmunogen: Full length Taq DNA polymerase
bulletSpecificity: Reacts with various commercial available Taq DNA polymerases (native or recombinant)
bulletIsotype: IgG2b
bulletClone number: 8C1
bulletPurity: 95% by SDS PAGE gel
bulletSize: 100 g (4 g/l)

>> Download Anti-Taq Monoclonal Antibody datasheet

 

Part #

Size

Price ($US)

Quantity

MA-029-0250 0.1 mg (25 l) $88.00


 

- Ordering Instructions -

Glycerol-free Anti-Taq Monoclonal Antibody (8C1C) 
100 g 

bulletDescription: Monoclonal Anti-Taq Antibody, Glycerol-free
bulletImmunogen: Full length Taq DNA polymerase
bulletSpecificity: Reacts with various commercial available Taq DNA polymerases (native or recombinant)
bulletApplications: Keep the Taq DNA polymerase from activated at storage conditions; eliminate the possible inhibitory effect by glycerol in the PCR amplification
bulletIsotype: IgG2b
bulletClone number: 8C1C
bulletPurity: 95% by SDS PAGE gel
bulletSize: 100 g (4 g/l)

>> Download Glycerol-free Anti-Taq Monoclonal Antibody datasheet

 

Part #

Size

Price ($US)

Quantity

MA-039-0100 0.1 mg (25 l) $95.00


 

- Ordering Instructions -

 

 

 

 

 

 

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